The iBlot 2 Gel Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably, within seven minutes, and without the need for liquid buffers. The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes and has comparable performance to traditional wet-transfer methods in a fraction of the time. The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).
iBlot 2 Gel Transfer Device features include:
Complete protein transfer in seven minutes or less
High detection sensitivity and even transfer
Increased blotting reliability and reproducibility
A simple, user-friendly system
Pre-programed with six voltage methods that allows blotting using different combinations of volts and time
Ability to create custom programs
Built-in tutorials and application notes
Transfer proteins from mini-, midi-, and E-PAGE-sized gels
How the iBlot 2 Dry Blotting System works
Buffer ion reservoirs are incorporated into the gel matrix of transfer stacks instead of buffer tanks or soaked papers. The high density of ions in the gel matrix enables rapid protein transfer. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen). With the iBlot 2 Dry Blotting System, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer. With the iBlot 2 system, there is no need to prepare buffers, pre-treat your gel, or clean up after blotting. The total preparation and run time is normally less than ten minutes per blot.
Specifications
For Use With (Equipment): iBlot
Gel Compatibility: Bolt Bis-Tris Plus Gels, E-PAGE Gels, Novex Midi Gels, NuPAGE Gels, Novex Mini Gels
Mode of Transfer: Dry
Running Dimension: Horizontal
Product Line: iBlot
Additional Prep Fees May Apply
