The Thermo Accela high-speed chromatographic system provides fast, efficient chromatographic separations over an expansive range of flow rates and pressures. Thermo Accela is designed to optimize performance of sub-two micron particle columns, providing seamless operation spanning conventional LC pressures from short LC columns, up to 15,000 psi for long-column separations of complex bio mixtures.
Includes:
Accela 1250 Pump
Accela PDA 80 Hz Detector (Part # 60057-60140)
Accela Autosampler (Part # 60057-60020)
Manufacture Specification
Accela 1250
Number of Solvents: 4
Pump Max. Pressure: 18130 psi
Flow Accuracy: .5 % at set point or +1L/min whichever is greater
Standard Flow Rate: 1 to 2000 L/min
Accela PDA Detector: Wavelength Range- 190800 nm at 1 nm increment
Wavelength Accuracy- 1 nm at 254 nm and 640 nm
Wavelength Calibration- Using Holmium oxide solution
Wavelength Resolution- 1.2 nm (512 pixel array)
Absorbance Non-Linearity- < 5% at 2.0 AU at 257 nm
Absorbance Range- -2.0 to +4.0 AU, 20-bit resolution
Drift- < 1 mAU/hr after warm-up at 254 nm at a stable temperature (1C)
Light Source- Pre-aligned Deuterium and Tungsten lamps
Rise Time- User selectable: 0, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, or 10 sec
Scan Rate- User selectable: 0.5, 1, 2, 4, 5, 10, 20, 40, or 80 Hz
Discrete Channels- Three wavelength selectable channels
Short-Term Noise (50 mm ow cell)- < 6 AU/cm at 254 nm at 5 Hz (20 Hz scan rate) < 1.5 uAU/cm at 254 nm at 5 Hz (40 Hz scan rate); typical < 0.3 uAU/cm at 254 nm at 5 Hz (80 Hz scan rate); typical
Warm-up Time- 90 min required to meet noise and drift specications
Cell Dimensions- 10 mm, 2 L LightPipe ow cell 50 mm, 10 L LightPipe ow cell
Cell Pressure Range- 01000 psi
Analog Outputs- 20-bit digital/analog conversion, unattenuated at 10 mV/AU, 100 mV/AU, or 1.0 V/AU
Diodes- 512
Diode Spacing- 1.2 nm
Accela Autosampler
Vials capacity: 200
Injection precision, @ 5 l: 1 %
Minimum sample volume, l: 1
Column oven, C: 4 - 95
Tray temperature control, C: 0 - 6
Additional Prep Fees May Apply
