The ABI 7300 is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual. This small but robust PCR takes up less space than the Applied Biosystems 7900HT. The ABI 7300 is great quantifying nucleic acid sequences without compromising data. In addition, the ABI 7300 works with a number of different dye choices. The Applied Biosystems 7300 Real Time PCR runs on its own software platform and quantitative results are available immediately, once PCR run is complete. The great thing about the ABI 7300 real-time PCR is theres no need to purify PCR, run any gels, or implement post PCR alterations. Complete Real-Time PCR runs in less than two hours with the ABI 7300 real time using tubes, 96 well plates, 8-strip tubes. The acceptable volume range is 20-100l which saves time when compare to manual PCR applicationsuch as RNase protection, Northern blotting.
The Applied Biosystems 7300 delivers four color detect and has the ability to run multiple applications including SNP genotyping, pathogen quantification, isothermal, gene expression analysis, along with plus/minus assays which use internal positive controls.
Manufacture Specifications:
Thermal cycling systemPeltier-based, 96-well block
Sample Ramp Rate 1.1C/sec
Peak Block Ramp Rate1.6C/sec
Temperature Range4C 100C
Temperature Accuracy 0.25C of setpoint / display temperature measured at 3 minutes after clock start
Temperature Uniformity 0.50C, 30 seconds after clock start
Optical system Single excitation, four emission filters, and CCD camera
Calibrated Dyes at Installation SYBR Green, FAM, JOE, NED, TAMRA, ROX
Passive Reference DyesROX or any calibrated dye
Data Collection Data collected in all 4 filters for all wells regardless of plate setup. Plate setup may
Quantitative PCR run time< 1 hour 50 minutes
Supported Volumes 20 100 L
Supported Consumables Standard optical 96 well plates
0.2ml tubes
Optical adhesive covers
Optical flat caps
8-strip 0.2ml tubes
**Please note that this instrument can include a Laptop AND a copy of the SDS Software
(END USER IS responsible for any and all validation and certifications) with purchase for an additional fee of $450.00. Contact us if you would like to add that to your order!
Additional Prep Fees May Apply
