The BD Biosciences LSR II is a multi-laser flow cytometer that utilizes fixed-alignment lasers that transmit light reflected by mirrors through a flow cell to user-defined octagon and trigon arrays. These fluorescence signals are collected and translated to electronic signals then converted to digital data. The BD LSR II utilizes innovative optics and digital electronics to provide highly flexible yet powerful performance with a greater sensitivity, which yields more information from each sample.
Manufacture Specification
Multilaser multiparameter analysis cytometer
Three lasers, 11 colors
Automatic compensation (compensation controls are still needed)
355 nm (UV): BUV737, BUV395, DAPI
405 nm (Violet): CFP, Alexa Fluor 430, Pacific Blue, Marina Blue, Alexa Fluor 405
488 nm (Blue): FITC, PE, PerCP, Pe-Cy5 (aka CyChrome), PI, PerCp-Cy5.5, PE-Cy7, GFP, PE-Texas Red
633 nm (Red): APC, APC-Cy7, Alexa Fluor 647, Alexa 680
Availability of UV gives a capability to perform Ca 2+ flux experiments.
Unlike a FACSCalibur, it is possible to use PE-Cy5 and APC together.
Additional Prep Fees May Apply.
